Coding

Part:BBa_I746918:Design

Designed by: Stefan Milde   Group: iGEM07_Cambridge   (2008-09-30)


mut3b GFP with 6-his tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

2 stop codons were removed from the mut3b coding sequence and reinserted after the 6-his tag.


Source

The part was obtained by PCR from mut3b GFP from the 2007 parts distribution.

References